腫瘤的發生發展是一個復雜的過程，涉及一系列遺傳和表觀遺傳的改變。這些改變使得細胞能夠擺脫正常生長調控信號（如外部環境和內部信號）的束縛，獲得自主增殖的能力。其中，錨定非依賴性生長（Anchorage-independent growth）是細胞惡性轉化的重要標志之一，而軟瓊脂集落形成實驗（Soft Agar Colony Formation Assay）被認為是檢測細胞惡性轉化的金標準。
軟瓊脂集落形成實驗通過在半固體培養基中培養細胞，觀察其是否能夠在無錨定條件下形成集落，從而判斷細胞的惡性轉化能力。然而，這種方法通常需要3-4周才能獲得結果，嚴重拖慢研究進度，并且由于依賴人工顯微鏡計數集落，難以統一判定導致結果偏差。最重要的是傳統軟瓊脂實驗無法回收活細胞，限制了后續研究的開展。
Cellbiolabs新一代CytoSelectTM檢測系統結合熒光定量技術和改良軟瓊脂配方，全面優化了實驗流程：
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CytoSelectTM細胞轉化檢測試劑盒通過高靈敏度的熒光檢測法定量細胞集落，實驗周期從3-4周縮短至1周內，顯著提高了研究效率，同時避免了人工計數的主觀誤差，尤其適合高通量樣本檢測。對于蛋白質/DNA芯片分析或癌癥疫苗開發等應用領域而言，使用專用改良軟瓊脂培養基可以輕松回收活化的轉化細胞供進一步培養和測試使用。
Fig 1?細胞活力檢測：按照實驗方案回收HeLa和293細胞培養6天，用臺盼藍法測定細胞活力。
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產品訂購信息：
|
貨號 |
品名 |
檢測方法 |
|
CBA-135 |
CytoSelect? 96-Well Cell Transformation Assay, Cell Recovery Compatible |
比色法 |
|
CBA-140 |
CytoSelect? 96-Well Cell Transformation Assay, Cell Recovery Compatible |
熒光法 |
|
CytoSelect? 96-Well Cell Transformation Assay, Soft Agar Colony Formation |
熒光法 |
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產品部分已發表文獻：
1. El Baba, R. et al. (2023). Polyploidy, EZH2 upregulation, and transformation in cytomegalovirusinfected human ovarian epithelial cells. Oncogene. doi: 10.1038/s41388-023-02813-4.
2. Hiroki, H. et al. (2023). Targeting Poly(ADP)ribose polymerase in BCR/ABL1-positive cells. SciRep. 13(1):7588. doi: 10.1038/s41598-023-33852-2.
3. El Baba, R. et al. (2023). EZH2-Myc driven glioblastoma elicited by cytomegalovirus infection ofhuman astrocytes. Oncogene. doi: 10.1038/s41388-023-02709-3.
4. Kantisin, S. et al. (2022). In utero arsenic exposure increases DNA damage and gene expressionchanges in umbilical cord mesenchymal stem cells (UC-MSCs) from newborns as well as in UCMSC differentiated hepatocytes. Toxicol Rep. doi: 10.1016/j.toxrep.2022.09.002.
5. Nehme, Z. et al. (2022). Polyploid giant cancer cells, EZH2 and Myc upregulation in mammaryepithelial cells infected with high-risk human cytomegalovirus. EBioMedicine. 80:104056. doi:10.1016/j.ebiom.2022.104056.
6. Kim, D.G. et al. (2022). AIMP2-DX2 provides therapeutic interface to control KRAS-driventumorigenesis. Nat Commun. 13(1):2572. doi: 10.1038/s41467-022-30149-2.
7. Buranarom, A. et al. (2021). Dichloromethane increases mutagenic DNA damage andtransformation ability in cholangiocytes and enhances metastatic potential in cholangiocarcinomacell lines. Chem Biol Interact. doi: 10.1016/j.cbi.2021.109580.
8. Nehme, Z. et al. (2021). Polyploid giant cancer cells, stemness and epithelial-mesenchymalplasticity elicited by human cytomegalovirus. Oncogene. doi: 10.1038/s41388-021-01715-7.
9. Andrade, F. et al. (2021). Polymeric micelles targeted against CD44v6 receptor increaseniclosamide efficacy against colorectal cancer stem cells and reduce circulating tumor cells in vivo.J Control Release. 331:198-212. doi: 10.1016/j.jconrel.2021.01.022.
10. Wakae, K. et al. (2020). EBV-LMP1 induces APOBEC3s and mitochondrial DNA hypermutationin nasopharyngeal cancer. Cancer Med. doi: 10.1002/cam4.3357.
11. Lv, W. et al. (2020). Reprogramming of Ovarian Granulosa Cells by YAP1 Leads to Developmentof High-Grade Cancer with Mesenchymal Lineage and Serous Features. Sci Bull. doi:10.1016/j.scib.2020.03.040.
12. Murata, M. et al. (2020). OVOL2-Mediated ZEB1 Downregulation May Prevent Promotion ofActinic Keratosis to Cutaneous Squamous Cell Carcinoma. J Clin Med. 9(3). pii: E618. doi:10.3390/jcm9030618.
13. Hernandez, D.M. et al. (2020). IPF pathogenesis is dependent upon TGFβ induction of IGF-1.FASEB J. doi: 10.1096/fj.201901719RR.
14. Sand, A. et al. (2019). WEE1 inhibitor, AZD1775, overcomes trastuzumab resistance by targetingcancer stem-like properties in HER2-positive breast cancer. Cancer Lett. 472:119-131. doi:10.1016/j.canlet.2019.12.023.
15. Paul, M. et al. (2022). Nitric-Oxide Synthase trafficking inducer (NOSTRIN) is an emerging negative regulator of colon cancer progression. BMC Cancer. 22(1):594. doi: 10.1186/s12885-022-09670-6.
16. Kondo, M. et al. (2021). Safety and efficacy of human juvenile chondrocyte-derived cell sheets forosteochondral defect treatment. NPJ Regen Med. 6(1):65. doi: 10.1038/s41536-021-00173-9.
17. van der Toorn, M. et al. (2018). The biological effects of long-term exposure of human bronchialepithelial cells to total particulate matter from a candidate modified-risk tobacco product. Toxicol In Vitro. 50:95-108. doi: 10.1016/j.tiv.2018.02.019.
18. Montalbano, M. et al. (2016). Modeling of hepatocytes proliferation isolated from proximal and distal zones from human hepatocellular carcinoma lesion. PLoS One. 11:e0153613.
19. Choi, B.Y. et al. (2023). Engineered Mesenchymal Stem Cells Over-Expressing BDNF Protect theBrain from Traumatic Brain Injury-Induced Neuronal Death, Neurological Deficits, and CognitiveImpairments. Pharmaceuticals (Basel). 16(3):436. doi: 10.3390/ph16030436.
20. Ikeda, J. et al. (2023). Hypoxia inducible factor‐1 activator munc‐18‐interacting protein 3 promotestumour progression in urothelial carcinoma. Clin Transl Disc. 3:e158. doi: 10.1002/ctd2.158.
21. Switzer, C.H. et al. (2022). NOS2 and S-nitrosothiol signaling induces DNA hypomethylation andLINE-1 retrotransposon expression. Proc Natl Acad Sci U S A. 119(21):e2200022119. doi:10.1073/pnas.2200022119.
22. Furuya, K. et al. (2022). Machine learning extracts oncogenic-specific γ-H2AX foci formationpattern upon genotoxic stress. Genes Cells. doi: 10.1111/gtc.13005.
23. Kim, M. et al. (2022). BRAFV600E Mutation Enhances Estrogen-Induced Metastatic Potential ofThyroid Cancer by Regulating the Expression of Estrogen Receptors. Endocrinol Metab (Seoul).37(6):879-890. doi: 10.3803/EnM.2022.1563.
24. Toh, P.J.Y. et al. (2022). Optogenetic control of YAP cellular localisation and function. EMBORep. doi: 10.15252/embr.202154401.
25. Lee, A.R. et al. (2022). Biomarker LEPRE1 induces pelitinib-specific drug responsiveness byregulating ABCG2 expression and tumor transition states in human leukemia and lung cancer. SciRep. 12(1):2928. doi: 10.1038/s41598-022-06621-w.
26. Wang, Y. et al. (2022). Long non-coding RNA OIP5-AS1 suppresses microRNA-92a to augmentproliferation and metastasis of ovarian cancer cells through upregulating ITGA6. J Ovarian Res.15(1):25. doi: 10.1186/s13048-021-00937-3.
27. Andriolo, G. et al. (2021). GMP-Grade Methods for Cardiac Progenitor Cells: Cell BankProduction and Quality Control. Methods Mol Biol. doi: 10.1007/7651_2020_286.
28. Tan, T.T. et al. (2021). Assessment of Tumorigenic Potential in Mesenchymal-Stem/Stromal-CellDerived Small Extracellular Vesicles (MSC-sEV). Pharmaceuticals. 14(4):345. doi:10.3390/ph14040345.
29. Lo, E.K.K. et al. (2021). Low dose of zearalenone elevated colon cancer cell growth through Gprotein-coupled estrogenic receptor. Sci Rep. 11(1):7403. doi: 10.1038/s41598-021-86788-w.
30. Park, S. et al. (2021). Cerebral Cavernous Malformation 1 Determines YAP/TAZ SignalingDependent Metastatic Hallmarks of Prostate Cancer Cells. Cancers (Basel). 13(5):1125. doi:10.3390/cancers13051125.
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參考文獻
1. El Baba, R. et al. (2023). Polyploidy, EZH2 upregulation, and transformation in cytomegalovirus infected human ovarian epithelial cells. Oncogene. doi: 10.1038/s41388-023-02813-4.
2. Hiroki, H. et al. (2023). Targeting Poly(ADP)ribose polymerase in BCR/ABL1-positive cells. Sci Rep. 13(1):7588. doi: 10.1038/s41598-023-33852-2.
3. El Baba, R. et al. (2023). EZH2-Myc driven glioblastoma elicited by cytomegalovirus infection of human astrocytes. Oncogene. doi: 10.1038/s41388-023-02709-3.
4. Kantisin, S. et al. (2022). In utero arsenic exposure increases DNA damage and gene expression changes in umbilical cord mesenchymal stem cells (UC-MSCs) from newborns as well as in UC-MSC differentiated hepatocytes. Toxicol Rep. doi: 10.1016/j.toxrep.2022.09.002.
5. Nehme, Z. et al. (2022). Polyploid giant cancer cells, EZH2 and Myc upregulation in mammary epithelial cells infected with high-risk human cytomegalovirus. EBioMedicine. 80:104056. doi: 10.1016/j.ebiom.2022.104056.
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Cell Biolabs公司坐落于美國加利福尼亞州圣地亞哥市，一直致力于開發生命科學研究領域的技術和工具，并將所開發的創新性技術成果商業化。Cell Biolabs孜孜不倦的完善產品，以期使細胞功能和疾病機制研究達到新高度。Cell Biolabs公司的產品獨具特色并且處于行業前沿水平，全球眾多大學、政府研究機構、生物、制藥企業的科研實驗室均在使用。
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北京西美杰是Cell Biolabs品牌中國授權代理，為用戶提供完善的技術支持與售后服務。如對產品感興趣歡迎撥打西美杰客服熱線400-050-4006或登錄網站www.gp82.cn了解更多信息。
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