The establishment of a cell bank can provide cells that are qualified through verification, consistent in quality, and capable of continuous and stable passage for the production of biological products. In accordance with the provisions of the Chinese Pharmacopoeia (2020 Edition), cell lines used for biological product production shall establish a three-tier cell bank system, including the Primary Cell Bank (PCB), Master Cell Bank (MCB), and Working Cell Bank (WCB). It is also required that cells maintain good viability after cryopreservation, and the viability of recovered cells (through regular sampling and thawing) shall not be less than 80%.

In addition, after the establishment of the cell bank, a comprehensive verification must be conducted on potential exogenous contaminating factors (including bacteria, fungi, mycoplasma, viruses, etc.) to eliminate possible potential contamination risks. Therefore, selecting a cell cryopreservation medium with excellent cryoprotective performance, well-defined components, and compliance with GMP production standards is an ideal solution for the cryopreservation of cell lines used in biological products.

Bambanker is a serum-free, animal-derived component-free, and chemically defined ready-to-use cell cryopreservation medium. It is applicable to nearly all types of cell lines, including primary cells, stem cells, lymphocytes, and conventional cell lines. This product has been cited in more than 50 peer-reviewed publications and has received consistent acclaim from users worldwide. Currently, the JCRB Cell Bank uses Bambanker to cryopreserve over 1,400 different cell lines, and the viability of cells after more than 5 years of cryopreservation remains above 80% upon recovery. Compared with the traditional cell cryopreservation method using serum-supplemented media, cryopreserving cells with Bambanker offers unparalleled advantages, mainly including:

· Adopting an animal-derived component-free formula with chemically defined components, which avoids contamination from uncertain sources such as mycoplasma and viruses.

· Enabling long-term and stable preservation of cells at low temperatures of -80°C or -196°C.

· Being a ready-to-use cell cryopreservation medium that requires no dilution or programmed cooling steps.

· Effectively improving the viability of cells after recovery.

· Compliant with GMP (Good Manufacturing Practice) production standards.

· Applicable to all cell lines.

· Boasting a long shelf life: 2 years of storage at 2-10°C.

Bambanker contains no serum components. Serum is associated with drawbacks such as undefined composition and significant batch-to-batch variation. If cells are cryopreserved in a serum-containing matrix, it not only leads to unstable cell viability after recovery but also may introduce other potential contaminating factors. In contrast, Bambanker adopts a chemically defined formula with clear components, which can efficiently protect cells while avoiding the aforementioned potential risks. Additionally, Bambanker is produced in compliance with GMP standards and equipped with a comprehensive quality management system, ensuring the consistency, reliability, and high quality of the product.

The usage method of Bambanker is also extremely simple. As shown in the figure below, it requires no programmed cooling steps and supports long-term stable preservation of cells at -80°C.

Bambanker Product Information

Beijing XMJ Technology Co., Ltd. is the authorized distributor of the Nippon Genetics brand in China. The 100ml specification of Bambanker Cryopreservation Medium is currently available in stock and selling well. If you are interested in the aforementioned product, please feel free to send email to info@xmjsci.com or visit the official website www.gp82.cn for more product information.